The station and all trains serving it are operated by South Western Railway.

Mortlake is the closest station to the finish of the Oxford-Cambridge University Boat Race.

Along with Richmond, it was not finished in time for a directors' special on 22 July 1846 and was still incomplete when the line opened due to delays in obtaining land.

Queen Victoria's Waiting Room The building next to Mortlake railway station – now occupied by a classic car showroom – houses Queen Victoria's old waiting room, built for her and Prince Albert as they frequented White Lodge in Richmond Park, where their family and later their son, Prince of Wales (later Edward VII ) lived.

It is considered to be the fourth most risky CCTV-crossing on Network Rail's Wessex Route.