The Mount Holly, Lumberton and Medford Railroad was a railway company in the United States.

It was incorporated in 1866 and opened its line in 1869, at which point it was leased by the Camden and Amboy Railroad.

[3] It began building its line between Mount Holly and Medford, New Jersey, in July 1869.

The line opened on December 20, 1869, at which point the Camden and Amboy Railroad leased the company.

[3] The Medford and Mount Holly Branch was not conveyed to Conrail in 1976 and was subsequently abandoned.