The Mount Holly, Lumberton and Medford Railroad was incorporated on April 2, 1866.

[1] Construction of its line between Mount Holly and Medford, New Jersey began in July 1869 and was completed on December 20, 1869.

At Mount Holly the line connected with the Pemberton Branch of the Camden and Burlington County Railroad.

[1] Passenger service between Medford and Mount Holly ended on November 27, 1926.

[7] The Medford and Mount Holly Branch was not conveyed to Conrail in 1976 and was subsequently abandoned.