Introduction of additional measurement laser wavelengths and first thin film analyses were performed in 2011 giving way to MP-SPR method.
This results in measurements of full SPR curves at multiple wavelengths providing additional information about structure and dynamic conformation of the film.
[4] The sensograms can be fitted using binding models to obtain kinetic parameters including on- and off-rates and affinity.
The full SPR curves are used to fit Fresnel equations to obtain thickness and refractive index of the layers.
In life sciences, the main applications focus on pharmaceutical development including small-molecule, antibody or nanoparticle interactions with target with a biomembrane[5] or with a living cell monolayer.