It features exhibits on the history of the Rio Grande Valley, as well as the rest of South Texas and North Eastern Mexican States Coahuila, Nuevo Leon, Tamaulipas.

[citation needed] The collections include a prehistoric mosasaur and an ice age mammoth.

In November of 2007, an exhibit of the "Citrus Era" and the turmoil of the Mexican Revolution was added along with recent area history.

The Margaret H. McAllen Memorial Archives house the Valley's largest collection of historical photographs, plus documents and maps, available for research.

[citation needed] The Museum Store offers South Texas history books and memorabilia of the Rio Grande Valley.