NY 328 parallels Seeley Creek, a tributary of the Chemung River, for its entire length.

The entirety of the 6.15-mile (9.90 km) route is part of Corridor U of the Appalachian Development Highway System.

NY 328 picks up where PA 328 leaves off at the Pennsylvania state line in the southwestern Chemung County town of Southport.

The route heads northeast, roughly paralleling Seeley Creek as it runs along the base of a large valley surrounding the waterway.

Here, NY 328 transitions from a two-lane roadway to a four-lane divided highway, a configuration that remains in place for the final 3 miles (5 km) of the route.

[18][19][20] The realignment coincided with the designation of the route as part of Corridor U of the Appalachian Development Highway System in 1965.