The route progresses eastward as a residential road through Tioga Junction, turning southeastward after Hillside Terrace.

After this curve, PA 328 leaves Tioga Junction a two-lane local road through woodlands.

[2] After the hamlet of Trowbridge, PA 328 remains a rural highway, turning to the northeast through the fields in the area.

Soon after, the route enters the census-designated place of Millerton and gains the name of Main Street.

PA 328 passes a mix of commercial businesses and residential homes until leaving Millerton after the intersection with Bly Road.