Coming from Aschaffenburg the line branches off towards Würzburg, about five kilometres northeast of Lohr, in the cadastral district of Nantenbach, in the community of Neuendorf.

The route is 11.3 kilometres long with continuous double track, electrified, equipped with the German train protection system, Linienzugbeeinflussung and running on many engineering structures: the 694.5 metre long viaduct over the Main, followed by the 3,941 m long Schönrain Tunnel (passing under the 5,528 m long Muhlberg Tunnel on the Hanover–Würzburg line) and another three tunnels.

The track rises 127 m from the Nantenbach junction at 156 m above sea level to Rohrbach operating station at 283 meters above sea level on a consistent grade of 1.25%.

The track is located for its full length in the Main-Spessart nature park.

[4] Construction started in March 1990[5] and the line was put into operation in June 1994.