Nature Reserve of Saint Barthélemy (Réserve Naturelle de Saint-Barthélemy) is a nature reserve of Saint Barthélemy (RNN 132), French West Indies, an overseas collectivity of France.

Founded in 1996, it covers 1,200 hectares in five sectors: Gros Îlets and Pain de Sucre off Gustavia harbour; the waters surrounding the islets Fourchue, Frégate and Île Toc Vers; and part of Colombier bay.

[2] It was established in 1996 with the objective of conserving coral reefs, sea grass beds and the marine life.

The land area is small and its xerophytic vegetation is grazed by goats, causing loss of nestling ground for 15 terrestrial birds.

The key bird species recorded here are: brown booby (Sula leucogaster); laughing gull (Leucophaeus atricilla); royal tern (Thalasseus maximus); and common tern (Sterna hirundo).