It features the five main characters from the games and cartoon: Mario, Luigi, Princess Toadstool (as the name Peach was not known in America at the time until Yoshi's Safari in 1993), Toad, and Bowser as "King Koopa".

He is an ill-willed character whom Tatanga—the villain of source game—could easily hypnotize into doing his bidding due to the bitterness in the man's cynical soul.

Simon Belmont and Mega Man are replaced by Samus Aran, the Metroid heroine who does not appear in the cartoon, and Mother Brain's second-in-command is Uranos, the demigod from Kid Icarus.

For unexplained reasons, Pit's toga is bleached yellow, and in most of the stories, Lana's dress is purple.

The comics retain some of the creative license taken by the cartoon's creators for elements of the original game (i.e., Metroid being a place, King Hippo being blue, Mother Brain's appearance).