Nourseothricin (NTC) is a member of the streptothricin-class of aminoglycoside antibiotics produced by Streptomyces species.

It is used as a selection marker for a wide range of organisms including bacteria, yeast, filamentous fungi, and plant cells.

[2] Streptothricin F is effective against highly drug-resistant gram-negative bacteria, including carbapenem-resistant E.

[3] NTC can be inactivated by nourseothricin N-acetyl transferase (NAT) from Streptomyces noursei, an enzyme that acetylates the beta-amino group of the beta-lysine residue of NTC.

[5] NTC is highly soluble in water (~ 1 g/mL) and stable in solution for 2 years at 4 °C.