Nuclear transfer

In rare instances, the newly constructed cell will divide normally, replicating the new DNA while remaining in a pluripotent state.

[1][2][3] Despite this, the low efficiency of the technique has prompted some researchers, notably Ian Wilmut, creator of Dolly the cloned sheep, to abandon it.

[4] Nuclear transfer is a delicate process that is a major hurdle in the development of cloning technology.

Microtubule inhibitors, such as nocodazole, are used to arrest the oocyte in M phase, during which its nuclear membrane is dissolved.

Currently unidentified reprogramming factors present in oocytes are capable of initiating a cascade of events that can reset the mature, specialized cell back to an undifferentiated, embryonic state.