Nutrient agar

Nutrient agar is a general-purpose solid medium supporting growth of a wide range of non-fastidious organisms.

It typically contains (mass/volume):[1] Nutrient broth has the same composition, but lacks agar.

Then they are cooled to around 50 °C (122 °F) and poured into Petri dishes which are covered immediately.

Once the dishes hold solidified agar, they are stored upside down and are often refrigerated until used.

Inoculation takes place on warm dishes rather than cool ones: if refrigerated for storage, the dishes must be rewarmed to room temperature prior to inoculation.