Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic and facultative anaerobic bacteria.
It is a general purpose medium that is routinely used to grow bacteria which tend to have high nutritional requirements (i.e., they are fastidious).
[1] Sterility test medium in USP and EP as well as for inocula preparation for CLSI standards.
[1] TSB is frequently used in commercial diagnostics in conjunction with the additive sodium thioglycolate which promotes growth of anaerobes.
[2] To prepare 1 liter of TSB, the following ingredients are dissolved under gentle heat.