Between Cygnet and Perrysburg, it is the relic of an old U.S. Route 25 (US 25) that passes through Bowling Green as largely a divided surface highway.

From Perrysburg it crosses the Fort Meigs Memorial (Maumee–Perrysburg) Bridge running concurrently with US 20 and then follows the Anthony Wayne Trail from Maumee into downtown Toledo.

The Maumee portion runs concurrently with US 24, while US 24 in greater Toledo takes, as far south as Perrysburg, the path of what used to be US 25, more distant from the newer Interstate 75 (I-75), having had its route switched with that of old US 25.

SR 25 heads westerly from its southern terminus at an interchange with I–75 in rural Wood County.

The route turns due north and becomes a four-lane divided highway, still closely paralleling I–75, before I–75 heads northeasterly.

The highway passes through the downtown business district of Bowling Green and an intersection with SR 64 (Wooster Street).

At Poe Road, SR 25 passes through commercial properties before leaving Bowling Green and becoming Dixie Highway.

The concurrency heads northwestward as a four-lane undivided highway passing through woodland and crossing over the Maumee River.

At Harvard Boulevard and Woodsdale Avenue, the route passes through the Toledo Zoo and under a set of railroad tracks.

After a couple blocks, Cherry Street meets southbound SR 25 at Spielbusch Avenue.

The parkway ends northeast of the interchange at Galena Street in a wooded area near the Toledo Correctional Institute.