The highway's northern terminus is at a T-intersection with SR 133 nearly 5.25 miles (8.45 km) southwest of Blanchester in the unincorporated community of Edenton.

All of SR 727 is nestled within the northeastern corner of Clermont County and follows the course of the Stonelick Creek for its entire length.

Almost immediately, it enters Wayne Township where it remains for the remainder of the route.

The route passes through Modest, and continues northeast through a mix of residential areas, farmlands, and forested areas until it curves sharply to the northwest at the border of Stonelick State Park.

SR 727 passes two park access roads before it crosses over the creek in front of the dam impounding Stonelick Lake.