Overgrowth's story takes place several years after the events of Lugaru and follows the same protagonist: Turner.

After avenging the murder of his family, killing the Alpha wolf, and killing the corrupt king Hickory, Turner refused to take Hickory's place on the throne, instead choosing to leave the monarchy headless and wander the island in search of some new purpose.

The game takes place on the island of Lugaru, an unknown number of years after the fall of the human race, indicated by the ruins of tall man-made structures overrun by vegetation.

Lugaru is a chaotic world, inhabited by anthropomorphic rabbits, wolves, rats, cats and dogs living at a pre-industrial level of technology.

Overgrowth is built on the gameplay of its predecessor, Lugaru,[3] and as such uses a hand-to-hand combat system that bases attacks and counters on timing and context using context-sensitive action buttons rather than different key combinations.