The pGLO plasmid was made famous by researchers in France who used it to produce a green fluorescent rabbit named Alba.
Other features on pGLO, like most other plasmids, include a selectable marker and an MCS (multiple cloning site) located at the end of the GFP gene.
[1][2][3] The GFP gene was first observed by Osamu Shimomura[4] and his team in 1962 while studying the jellyfish Aequorea victoria that have a ring of blue light under their umbrella.
In 1994 Marty Chalfie[4] and his team were able to successfully create bacteria and round worms that expressed the GFP protein.
The three scientists hold the Nobel Prize in Chemistry for 2008[6] for the discovery and development of the green fluorescent protein, GFP.