[4] PRIME has been developed in order to achieve a high labeling specificity comparable to fluorescent proteins with small molecules.
[1][4][5] “Functional group handle” indicates a bridge molecule connecting a LAP tag to a fluorescent probe or fluorophore.
Fluorescent probe reacts with the “functional group handle” connected to the tag, and ultimately labels the protein of interest.
[1] It is recommended that the experimenters perform control experiments in order to make sure that the tagged recombinant protein functions properly.
[1] Experimenters are also required to obtain the right balance between maximal signal of fluorescence and minimal disruption of cellular function.