Paraguay competed in the 2021 Junior Pan American Games in Cali–Valle, Colombia from 25 November to 5 December 2021.

Flag bearers at the opening ceremony were swimmer Luana Alonso and judoka Ángel Gini.

[2] At the closing ceremony flag bearers were gold medal-winning rower Javier Insfran and bronze medal-winning fencer Montserrat Viveros.