The conglomerate first appeared in The Superior Spider-Man #20 (October 2013), created by Dan Slott, Christos Gage, and Humberto Ramos, and ended during Secret Empire.

With the help of Dr. Elias Wirtham, Otto made artificial legs for Flash Thompson to replace the ones he lost when he was fighting in the Iraq War.

[4] In the aftermath of the fight against the Goblin Nation, Peter himself took up the reins to manage the company and publicly announced that he was no longer "working with Spider-Man".

[5][6] Peter soon announced that the company would be putting its cybernetics project on hold in order to focus on the capture, curing and depowering of Electro, and to develop a new super-villain prison.

[14] Parker Industries later set up its temporary headquarters in the renovated Baxter Building where Clayton Cole and Molly are working, which caused Human Torch to retaliate.

During this time, it is revealed that Harry Osborn is also working for Parker Industries' New York office while using his mother's maiden name of Lyman.

[15] Parker Industries worker Lian Tang is secretly in league with Scorpio where she provided him with security codes in exchange for medicine to give to her mother who was sick with cancer.

[16] It has since been revealed that the Zodiac have actually completely infiltrated Parker Industries, using its resources and funding to support their own agenda as one of their members is a CEO of one of the company's branches.

[22] During the Beyond storyline after helping to bring Peter Parker out of a coma that the U-Foes put him in, Doctor Octopus attacks the satellite location of Beyond Corporation's shell corporation Infinite Solutions and finds that they were taking assets from the defunct Parker Industries, their use of Ben Reilly's incarnation of Spider-Man, and the copying of Doctor Octopus' Spider-Man technology.