Pavel Tlustoš (born 27 February 1955 in Nové Město na Moravě, Czechoslovakia) is a Czech agricultural chemist.
He worked as a chemical technician for the Chirana company, before joining CULS as a research assistant in agrochemistry and plant nutrition.
He began his PhD study in 1984 and defended his thesis Environmental fate of slow release fertilizers at the Czech Agricultural University in Prague in 1989.
He received his Venia legendi in Agrochemistry and Plant Nutrition and a professorship of CULS from Czech president Vaclav Havel in 2002.
He is also a paid member of numerous international journals about soil chemistry, plant nutrition and environmental research.