PA 310 begins at an intersection with US 119 in the borough of Punxsutawney, heading north on two-lane undivided Elk Run Avenue.

The road runs through commercial areas before crossing a Buffalo and Pittsburgh Railroad line at-grade and passing homes.

The road enters McCalmont Township and becomes Main Street, turning northeast and running through the residential community of Anita.

PA 310 heads northeast into Winslow Township and runs through farmland and woodland with occasional residences as an unnamed road.

The route continues northeast through more rural areas before heading into the borough of Reynoldsville and becoming Bradford Street, passing homes.