According to the British historian of medicine Edward Withington, Marchetti was one the most important Italian surgeons of the seventeenth century.

[1] Pietro Marchetti was professor of anatomy at Padua, where he was born, and where he continued to teach from 1652 until 1669, when he was allowed to resign his chair to his son Antonio.

A famed anatomist, Domenico Marchetti was a fervent supporter of Harvey's theory of blood circulation, and a fierce opponent of Riolan.

In 1652 he demonstrated, in the presence of Thomas Bartholin, that liquid injected into the arteries emerged by the veins.

[6] Domenico Marchetti was the author of a good compendium of anatomy, according to the judgment of Haller, which went through several editions, under the title of "Anatomia, cui Responsiones ad Riolanum, Anatomicum Parisiensem, in ipsius animadversionibus contra Veslingium, additae sunt", Padua, 1652.