As in all Plasmodium species, P. floridense has both vertebrate and insect hosts.

The vertebrate hosts for this parasite are lizards.

Schizonts are 1.5 -2.0 times the size of the nucleus of an uninfected erythrocyte.

This organism is found in an area stretching continuously from the southern United States to Panama.

[1] The prevalence of infection in Anolis sagrei in Florida is high (46%) but the median parasitaemia in infected hosts is low (0.3%).