On April 16, 2001, Playhouse Disney received a new rebrand produced by motion graphics company Beehive;[3] actress Allyce Beasley began serving as the U.S. block's promo announcer at this time, a capacity she would hold until March 30, 2007, being replaced by Margit Furseth.

Episodes from Disney Channel's original series were aired during the late morning and early afternoon hours.

[8][9] The Playhouse Disney block ended its 14-year run on February 13, 2011, with the last program to air being an episode of the short series Handy Manny's School for Tools at 8:55 a.m. Eastern Time.

The Disney Junior block debuted on February 14, 2011,[10] with the Little Einsteins episode "Fire Truck Rocket" as its first program.

[11][12][13][failed verification] Several former Playhouse Disney series were carried over to the relaunched block including Mickey Mouse Clubhouse, Special Agent Oso, Imagination Movers, Handy Manny, and Little Einsteins.

With the relaunch of the block, the block's mascots Ooh and Aah were retired and several of its older programs were entirely discontinued (however, Ooh and Aah & You was later briefly available on the Disney Junior website as a part of the Fan Favorites week of July 18, 2011 and was also later carried in reruns on the Disney Junior cable channel).

The 24-hour Disney Junior cable channel debuted on March 23, 2012, with the Mickey Mouse Clubhouse episode "Mickey's Big Surprise" as its first program, mainly featuring a mix of original series and programs held over from the Playhouse Disney library (which largely aired as part of the channel's overnight schedule until mid-2014 when over time, more Playhouse Disney shows were taken off the air completely after premiering their series finales and ceased to air in reruns).

[28] On April 4, 2009, Egmont Group launched a companion Playhouse Disney magazine in the United Kingdom & Republic of Ireland that focused on the channel's four most popular shows: Mickey Mouse Clubhouse, My Friends Tigger & Pooh, Handy Manny and Little Einsteins.