The Polly Hill Arboretum includes 8 ha (20 acres) under cultivation, with an additional 16 ha (40 acres) of native woodland, located on Martha's Vineyard at 809 State Road, West Tisbury, Massachusetts, United States.

In 1687, Henry Luce, one of the first English settlers on Martha's Vineyard purchased 400 acres (1.6 km2) of land in the island's center from the natives.

The Arboretum now contains North Tisbury azaleas, witch-hazels, winter hazels, camellias, magnolias, stuartias, conifers, and deciduous and evergreen hollies, and incorporates a dogwood avenue and a pleached hornbeam arbor.

Polly Hill plant introductions include varieties of Cornus kousa, Ilex, Stewartia, and about forty Rhododendrons.

Polly Hill also contains several Araucaria araucana, commonly called monkey puzzle trees, which are endangered in their native Argentina and Chile.