[2] Although designed as a VR game, Polybius is fully playable in 2D on a standard PlayStation 4, and also includes support for 3D television output.

[4] In early marketing, co-author Jeff Minter claimed to have been permitted to play the original Polybius arcade machine in a warehouse in Basingstoke.

[6] He said it was instead based on his interest in the ability of video games to induce the psychological state of flow (also called "being in the zone")[7] and his own observations of positive psychological effects as a result of playing VR games, most notably the unreleased VR version of TxK.

[8] On July 13, 2017, industrial rock band Nine Inch Nails released a music video for the song "Less Than", featuring a woman playing Polybius.

[9] Llamasoft later described the version of the game used in the video, a custom build running on the PC with user configurable effect sequencing.