Polystachyinae is a subtribe within the tribe Vandeae in family Orchidaceae.

The type genus of this subtribe is Polystachya.

[2] Members of this group are epiphytic and are distinguished from the other subtribes in the tribe Vandeae by their sympodial growth habit and the presence of four pollinia.

Pollination is mostly by small species of bees, however some species (e.g. Polystachya concrete) exhibit auto-pollination.

:[4] Aeridinae Angraecinae (including Aerangidinae) Adrorhizinae Polystachyinae