Portrait of a Seated Woman with a Handkerchief is a painting at the Art Gallery of Ontario, in Toronto.

The style is similar to the surviving works of Carel Fabritius, one of Rembrandt's most skilled pupils.

Aaltje van Uylenburgh, a cousin of Rembrandt's wife Saskia, is the most common conjecture.

The pose of the sitter suggests the painting was paired with a portrait of the woman's husband.

It today hangs in Cologne and is believed to depict J.C. Sylvius, van Uylenburgh's husband.