Responsibility for the depot fell upon the United States Army Ordnance Corps, and the first civilians were hired in 1942 as operations began.

In 1951 the depot assumed responsibility to distribute U.S. Air Force ammunition for an eight-state area, as well as storage of strategic and critical materials for the General Services Administration (GSA).

Responsibility for the Depot shifted from Tooele, to the US Army Materiel Command headquartered at Fort Belvoir, Virginia.

The empty bunkers and warehouses which used to store the non-chemical munitions and other supplies as well as repair and manufacturing facilities were turned over to the Pueblo Depot Activity Development Authority who currently offer these properties for civilian lease purposes.

Upon receipt of these munitions for secure storage, PUDA became one of eight installations within the United States where chemical weapons are stored.