Widely tropical-subtropical marine plankton, pelagic inhabitants of deep waters (lower mesopelagic to upper bathypelagic environment).

Phylogenetic tree base on morphology and fossil records shows that Pulleniatina evolved two lineages separated at Late Miocene (N17 zone), extant species are the obliquiloculata lineage, P. obliquiloculata separated from P. praecursor at Early- middle Pliocene.

Phylogenetic tree base on small sub-unit ribosomal DNA (SSU rDNA) shows that P. obliquiloculata has closely relationship with Neogloboquadrina.

There some coiling direction transition event happens after Pulleniatina differentiation, the cause and detailed mechanism are unknown, but it can still be used to assist in the judgment of geological age.

[5] Base on the nitrogen isotopic composition between two amino acids (glutamic acid and phenylalanine), the result shoes that P. obliquiloculata is omnivorous with herbivorous dependence, food source maybe contents more phytoplankton than zooplankton.