The league rules include a tie-breaker penalty shootout, unlimited substitutions, and the implementation of secret weapons.

[3] Ibai Llanos of Porcinos, was the first Kings League chairperson to confirm the creation of his women's team, Porcinas.

[5][6][7] While the Kings League's first season saw involvement from former professional footballers, the Queens League also includes current professionals, particularly in the organisation of teams: FC Barcelona Femení and Switzerland player Ana-Maria Crnogorčević is part of the staff of Saiyans FC;[8] her club teammates Patricia Guijarro, Clàudia Pina, and Sandra Paños are all ambassadors of teams; as are Spain player Jennifer Hermoso and Chile player Christiane Endler.

[4][12] Mexican sports newspaper Récord wrote upon the announcement of the Queens League that it would be important in promoting gender equality in the world of video gaming.

The twelve teams will each face each other once in regular season, with six matches taking place consecutively on each matchday (typically, Saturdays).

At the end of each regular season, the eight best-placed teams will face each other in the play-offs, which takes place as a seeded bracket.

If the scores are tied at the end of a match, a penalty shoot-out tiebreaker will be used, with players taking the shots from the halfway line.

Team staff have to remain seated on the bench throughout the match except for giving instructions to players or using the button to enact different special rules.