The Soviet training facility was created to produce highly specialized spies, including Black Widows Natasha Romanova and Yelena Belova.

Furthermore, the girls received a special treatment designed by biochemist Lyudmila Kudrin that allows them, for decades, to remain young, healthy and resilient at superhuman levels.

[2] Natasha Romanova, weary of espionage and adventure, retires to Arizona but is targeted, as were the other Black Widow graduates of the Red Room, by the North Institute, on behalf of the Gynacon corporation.

She discovers the Black Widows are being hunted because Gynacon, having purchased Russian biotechnology from Red Room's successor agency 2R, wants all prior users of the technology dead.

After killing Gynacon CEO Ian McMasters, she clashes with operatives of multiple governments to help Sally Anne Carter, a girl Natasha befriended in her investigations, whom she rescued with help from Daredevil and Yelena Belova.

[5] The All-New, All-Different Marvel was revealed that Hank Pym's daughter Nadia van Dyne through first wife Maria Trovaya was raised in the Red Room.