Agami subsequently moved to the Weizmann Institute of Science (WIS) in Israel to obtain his master's degree in the Department of Biophysics studying spliced leader RNA in Leishmania parasites.
Subsequently, he moved to the Department of Molecular Genetics at the WIS where he obtained his Ph.D. in 1999 under Yosef Shaul with a thesis titled: “Cell cycle and apoptosis control induced by the tyrosine kinase c-Abl”.
With the development of Ribosome profiling and CRISPR-Cas9 technologies, he further studied transcriptional Enhancers and mRNA translation, mostly focusing on enhancerRNAs and predicting metabolic changes in cancer.
Since 2008 Agami is also a full Professor at the Department of Molecular Genetics at Erasmus Medical Centre Rotterdam University, The Netherlands.
[3][4] His research greatly contributed to the fields of miRNAs, RNA binding proteins, Alternative cleavage and polyadenylation, non-coding RNAs, and mRNA translation.