Rodolfo Gambini (21 September 1855 – 8 March 1928) was an Italian painter, active mainly in Milan, Lombardy, Piedmont, and Liguria.

He frescoed an Ascent of Christ for the apse for the Duomo (Collegiata of San Lorenzo) of Voghera.

He painted for the parish church of San Nicolò in Pozzolo Formigaro.

He painted in the church of the Sacro Cuore in the Abbey of Oulx.

He painted in the church of Santa Maria Canale, Tortona.