The Roman Catholic Diocese of Atambua (Latin: Atambuen(sis)) is a Latin suffragan diocese in the ecclesiastical province of the Metropolitan of Kupang, in Indonesia, yet still dependent of the Roman Congregation for the Evangelization of Peoples.

Its cathedral episcopal see is Katedral Santa Maria Imakulata (dedicated to Mary Immaculate) located in the city of Atambua.

(all Roman rite, till 2007 members of a Latin missionary congregation)

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