It was established in 2016 as the women's section of Empoli, following an acquisition of the sporting license of Castelfranco.

Following a collaboration in 2015–16 with Castelfranco, the management of Empoli decided to form a women's football club in June 2016, buying 51% of the shares of the Castelfranco di Sotto club, and registering the team under the name of Empoli Ladies FBC.

[3] In the same season Empoli reached the semi-finals of the Coppa Italia, where they were eliminated by eventual champions Fiorentina.

[5] The 2018–19 season saw Empoli play in the first Serie B championship organized in a single group.

[6] At the end of the 2021–22 season, which ended with a ninth-place finish in the Serie A and a semi-finals finish in the Coppa Italia, Empoli decided to sell the Empoli Ladies company to Parma, continuing to operate in the youth sector.