When the Jesuits were removed from the Viceroyalty of the Río de la Plata, the Franciscan friars moved to the chapel at San Miguel del Carcarañal.

In 1790 they sought to establish another one more distant from the Parana river, and governor Félix Aldao gave them the current location.

Manuel Belgrano visited it in 1811, in his return from the Paraguay campaign, and the chapel helped him the following year to build the "Independencia" and "Libertad" batteries at modern Rosario.

José de San Martín followed a royalist ship navigating the Parana, and hid his troops inside the convent.

], mayor of San Lorenzo, made an agreement in 2008 with the Argentine Armed Forces so that the Regiment of Mounted Grenadiers have a permanent presence in the site.