Sant'Elena, also sometimes called Santa Lena, is a Gothic-style, Roman Catholic church at the extreme east end of the sestiere of Castello in the City of Venice, Italy.

This site was once the island of Sant'Elena, which was not then accessible by foot from the city; it now represents the east end of Venice.

The first chapel at this location was built by Augustinian monks in 1028 and dedicated to Saint Helene from Auxerre.

The main altarpiece, depicting the Adoration of the Magi with Saint Helena, was relocated to the Pinacoteca di Brera in Milan during the Napoleonic era, and remains there today.

The main altarpiece is a copy of that found in the Church of Sette Santi Fondatori in Florence.