After the Jesuits were expelled in 1873, the monastery was seized by the state, and Sant'Eusebio eventually became a parish church staffed by diocesan clergy.

The small piazza outside the church hosts an annual blessing for the animals on the 17 January – the feast of St. Anthony the Abbot.

The tradition dates back to 1437 and was transferred to Sant'Eusebio in the early 20th century from the nearby Church of Sant’Antonio Abate.

The present design dates to 1600 work by Onorio Longhi, who restored the presbytery, main altar, and choir.

The main altar has custody of the relics of St Eusebius of Rome,[5] who is supposed to have commissioned and financed construction of the church in the 4th century.