Santa Rosa (Spanish pronunciation: [ˈsanta ˈrosa]) is a city and district located in the Misiones department in Paraguay.

Santa Rosa stands on a hill located 257 kilometres (160 mi) south of Asunción on Route I "Mariscal Francisco Solano Lopez".

Santa Rosa has a population of 20,306 inhabitants, of whom 10,582 are men and 9,723 are women, according to projections by the General Directorate of Statistics, Census and Surveys.

To this day the bell is still in use, as part of the newer temple built on the site.

Santa Rosa is an agricultural area, with large cattle ranches.