It runs from the Alberta border where it becomes Highway 13 to intersection of Circle Drive and 22nd Street in Saskatoon.

It is approximately 250 kilometres (160 mi) long.

Prior to 1976, Highway 14 ran the width of the province, continuing east from Saskatoon to the Manitoba border where it transitioned to PTH 4.

Six years later, in 1976, these two sections were re-designated as Highway 16 so that the Yellowhead Highway would carry one number through the four Western provinces (Manitoba would follow suit the next year, redesignating PTH 4 to PTH 16).

Major communities that Highway 14 passes through are Macklin, Unity, Wilke, Biggar, Asquith, and Saskatoon.