Segregating sites are positions which show differences (polymorphisms) between related genes in a sequence alignment (are not conserved).
[1] Segregating sites include conservative, semi-conservative and non-conservative mutations.
The proportion of segregating sites within a gene is an important statistic in population genetics since it can be used to estimate mutation rate assuming no selection.
For example it is used to calculate the Tajima's D neutral evolution statistic.
This genetics article is a stub.