They include almost all hero characters from both the main novel series (Gourry Gabriev, Amelia, Zelgadiss, Sylphiel, etc.)

After finishing the main game, the player can also select three characters (with the fourth still automatically assigned) to play in a bonus scenario.

The game, released about a year before the first season of the first anime series, follows the light novels' continuity.

When introducing the manga Slayers: Knight of Aqualord, Slayers creator and the game's co-writer Hajime Kanzaka described several of the alternative continuities across mediums, mentioning this game as one of them taking place after the first arc of the novel series, namely after the eighth novel.

The game begins with a man finding an unconscious Lina Inverse and taking her back to his village.

Eventually, Lina and her companions learn that the Greater Beast Zelas Metallium has been creating copies of her.