It is located in the former records vault building at the former Southern Pacific Depot, which was renovated by the City of Tucson in 2004.
The museum offers community events, downtown historic walking tours (October–March), railroad safety education, an oral history program and an active collections department preserving artifacts pertaining to transportation in southern Arizona.
Every year in March, the Southern Arizona Transportation Museum, located in downtown Tucson, holds the Silver Spike Festival.
The 2014 event, had such notable guests as Mayor Jonathan Rothschild and Arizona Daily Star newspaper columnist David Leighton.
Tully, and Robert Leatherwood) of Tucson and music from the 4th United States Cavalry Regiment Band.