[1] [2] On November 8, 2008, Marvel EIC Joe Quesada confirmed that Spider-Girl would become a feature in the monthly anthology magazine Amazing Spider-Man Family.

2 #12 (October 2024) introduced the supervillain Funhouse (Maka Akana), the villainous protégé of Bullseye, who aiming to become Spider-Boy's nemesis, temporarily takes the name "Spider-Girl" to mock him while the two partake in a tournament.

The chimeria genetic daughter of Gwen Stacy, Ana Soria, and Scott Summers, grown by the Jackal using DNA stolen from Mr. Sinister, she has the ability to transform into a giant spider with optical lasers.

She is later revealed to be a Hawaiian mutant named Makawalu "Maka" Akana who possesses the ability to duplicate superpowers when she originally operated as Funhouse.

", Betty Brant is one of three candidates – along with Flash Thompson and John Jameson – who is bitten by the radioactive spider which gave Spider-Man his powers.

After confiding in Peter, and with his assistance, she begins to fight crime under the name "The Amazing Spider-Girl", with a mask similar to Spider-Man's but a very different costume.

This version, known by various names, is a gender-swapped clone of the Peter Parker of the Ultimate Universe, with all of his memories, from their perspective having gone to sleep one day a boy and woken up the next as a girl.

[15] The character appears in the "Spider-Verse" and Spider-Geddon storylines, now referred with her father's surname as Ashley Barton, and alternately referred to as "Spider-Girl" and "Spider-Woman" due to the family-friendly nature of the narrative, and is among the spider-powered characters who are recruited by The Superior Spider-Man (Doctor Octopus's mind in Peter Parker's body) to help fight the Inheritors, before returning to the Wastelands in "Venomverse" and "Old Man Quill".

[19] An alternate universe variant of May Parker/Spider-Girl originating from Iron Man 2020's timeline appears in the novel Time's Arrow 3: The Future by Tom DeFalco and Rosemary Edghill.