Staffolo is a comune (municipality) in the Province of Ancona in the Italian region Marche, located about 35 kilometres (22 mi) southwest of Ancona.

As of 31 December 2004, it had a population of 2,304 and an area of 27.6 square kilometres (10.7 sq mi).

[3] Staffolo borders the following municipalities: Apiro, Cingoli, Cupramontana, Jesi, San Paolo di Jesi.

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