The Squadron was deployed to Rota, Spain on January 28, 1964, from which it conducted routine deterrent patrols.
Following treaty renegotiations between the United States and Spain in 1975, Squadron 16 withdrew from Rota and moved to the Kings Bay Naval Base in southeastern Georgia.
[2] The Squadron was deactivated on June 25, 1994 as part of the draw-down in US submarine forces following the Cold War.
[2] Submarine Squadron 16 was reactivated on August 8, 1997 as part of a reorganization within the Navy to provide better support to its smaller units.
For Squadron 16, this was to "focus on the emerging operation SSGN multi-mission capability and [to] build on the excellence already established in strike warfare and special operations forces delivery and support, among many other missions while supporting SSBNs during and after overhaul periods.