In a small room at the former Air Force base known as Dale Mabry Field, five men and one woman officially formed Tallahassee Memorial Hospital.

Tallahassee Memorial gained national recognition in 1954 for its effective handling of a polio-like virus that hit Florida's Big Bend and on September 10, 1958 TMH expanded with the addition of a new wing housing an emergency department, a medical floor, obstetrics service, and surgical services.

From its founding until the late 1960s, Tallahassee Memorial did not admit black patients, who were treated at Florida A&M Hospital.

In 1978 The TMH Auxiliary provided 40,932 hours and purchased a heart-lung machine for $24,460 and a portable image intensifier for $43,500.

The Women's Pavilion features the region's only Level III Neonatal Intensive Care Unit.